Najmeh Maleki; Mehdi Dadmehr; Mohammad Ali Karimi
Abstract
In this research, a comparison was made between the concentration of secondary metabolites of Hibiscus sabdariffa content in its leaves and induced transgenic hairy roots. The levels of anthocyanins and flavonoids and total phenol were measured in both samples. In the next step, polyphenols were measured ...
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In this research, a comparison was made between the concentration of secondary metabolites of Hibiscus sabdariffa content in its leaves and induced transgenic hairy roots. The levels of anthocyanins and flavonoids and total phenol were measured in both samples. In the next step, polyphenols were measured by HPLC. The concentration of catechin flavonoids in the transgenic hairy root extract was 22.8 mg/g dry matter which was several times higher than that of 0.9 mg/g in leaf extract of dry matter. The obtained results clearly demonstrated that, the rate of concentration of secondary metabolites during the process of induction of transgenic hairy root was increased. Then, the extracts of leaf and transgenic hairy root samples at various concentrations of 0, 25, 50, 50, 100, 200, 400 mg / ml were added to HepG2 liver cancer cell lines and the percentage of cell survival was calculated by MTT method. The cell survival rate in the sample treated with transgenic hairy roots samples was less than the leaf extract samples, which was most likely due to the higher concentration of Catechin in the transgenic hairy roots extract. The best concentrations of extracts of transgenic hairy root extract showed that, the highest percentage of cell apoptosis was 100 mg/ ml.
